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INVENTION
Patent of the Russian Federation RU2183056
METHOD OF STIMULATION OF THE GROWTH OF CULTURE OF THE HAIR OF THE ORDINARY
The name of the inventor: Evdokimova OA; Polish SV; Aksenovskaya V.E .; Kashapova LA; Maneshin VV; Usacheva R.V.
The name of the patent holder: Voronezh State Agrarian University. K.D. Glinka
Address for correspondence: 394087, Voronezh, ul. Michurina, 1, VGAU, Patent Department
Date of commencement of the patent: 2000.02.03
The invention relates to stimulating the growth of oyster culture and solves the problem of increasing the efficiency of the method of growing oyster mushroom by shortening the duration of cultivation and enhancing the adaptive properties of the culture. This is achieved by introducing an immunocytophyte into the culture medium for their growth at a concentration of 5.2 × 10 -5 mg / ml of the active ingredient, the stimulant being introduced at the initial stage of obtaining the inoculum, namely, the agar medium.
DESCRIPTION OF THE INVENTION
The invention relates to a growth promoter used to increase the growth rate of oyster culture and increase yield.
Methods for increasing the productivity of oyster mushrooms are known by adding readily available sources of carbon and nitrogen to the substrate, such as molasses, bran, meal, etc. (1).
However, this enrichment of the nutrient medium leads to an increase in the likelihood of contamination of the substrate with a competitive microflora in the growing process.
A method for stimulating the growth of fungi, in particular champignons (chosen as a prototype), is known by introducing a proteinaceous additive in a concentration of 10-50 g per 1 kg of substrate at the stage of their growth. In this case, the additive is introduced into the substrate with sprouted mycelium or mixed with the substrate and champignon seedling when sowing (2).
Protein supplements are nutritional supplements, and are not specific growth stimulants. In addition, the addition of a nutritional supplement not only affects the mycelium of the mushrooms, but also the contention of competitors that are present in the substrate.
The invention solves the problem of increasing the efficiency of the method of growing oyster mushroom by shortening the duration of cultivation and enhancing the adaptive properties of the culture. These factors are of great importance in obtaining a seed of oyster culture, which includes 2 stages: 1) growing on an agarized nutrient medium, 2) growing the cereal seed (wheat, rye, oats, barley) of the seed inoculum For the production of oyster mushrooms in production conditions. In the process of transferring culture from a rich substrate to a poor one (from the wort to the grain and from the grain to the straw), the adaptation of the enzyme systems of the fungus to a new substrate takes place, which requires some time (lag period), and if the lag- The period is prolonged, the probability of infection of the seed with a competitive micro flora increases, the spores of which may be present in cereals and straw.
The solution of the stated task is achieved by the fact that in a method for stimulating growth of oyster mushroom, including the introduction of a growth stimulant into the growth medium, the immunocytophyte is used as the latter in a concentration of 5.2 × 10 -5 mg / ml of active ingredient (DV), the stimulant being introduced At the initial stage of obtaining the inoculum, namely, into the agarized nutrient medium.
The immunocytophyte is based on ethyl arachidonic acid - according to ISO, the structural formula of which is C 22 H 36 O 2 (3).
It is known that the producers of arachidonic acid synthesis are mushrooms of the Phycomycetes class. The addition of an immunocytophyte to the inoculum (wort agar) at a certain concentration results in an increase in the growth rate of oyster mushrooms on all types of substrates used (wort agar, grain, straw) and an increase in the yield of oyster mushrooms.
EXAMPLE 1 Control 1. A culture of oyster mushroom (Pleurotus ostreatus) is grown in a Petri dish on an agar medium (4-8% by Billing), pH 7-7.5; At t = 25 ° C. A piece of agar medium with a culture of 5 cm radius is used as the inoculum, which is placed in the center of the Petri dish with the medium. The culture is incubated in a thermostat until the cup surface is completely overgrown. The duration of cultivation is 11 days (264 hours), the growth rate is 0.45 cm / day.
EXAMPLE 2 EXPERIMENT 1. Growth of oyster mushroom is carried out under the same conditions as the control (Example 1), only the immunocytophyte solution in the concentration 5.2 · 10 -2 mg / ml DV is added to the agar medium. The duration of cultivation is 17 days (408 h). The maximum fouling radius is 4.5 cm, the growth rate is 0.26 cm / day. Thus, it can be concluded that there is an inhibition of culture growth.
EXAMPLE 3 EXPERIMENT 2. Growth of oyster mushroom is carried out under the same conditions as the control (Example 1), only the immunocytophyte solution in the concentration 5.2 · 10 -11 mg / ml DV is added to the agar medium. The duration of cultivation is 11 days (264 hours), the growth rate is 0.45 cm / day. Thus, it can be concluded that there are no significant changes in comparison with the control.
EXAMPLE 4 EXPERIMENT 3. Growth of oyster mushroom was carried out under the same conditions as the control (Example 1), only an agarose solution was added in 5.2 · 10 -5 mg / ml DV. The duration of cultivation is 6 days (144 hours), the growth rate of the culture is 0.83 cm / day.
Example 5. Control 2. Preparation of a grain mycelium. Grain cereals (wheat, barley, rye, oats) are washed, boiled, mixed with gypsum and chalk, covered in glass bottles with a capacity of 0.5 liters. The height of the grain layer is 19 cm. It is sterilized at 120 ° C for 1.5 hours. After cooling, the grain is inoculated with the seed material obtained in Example 1 (1/5 of the agarized surface of the cup per bottle). The seed inoculum is grown at t = 22-25 ° C. Cultivation is carried out until the contents of the bottle become completely overgrown. The duration of cultivation is 18 days (432 hours), the growth rate is 1.06 cm / day.
EXAMPLE 6 EXPERIMENT 4. Grain mycelium is grown under the same conditions as in the control (Example 5), only as a seed material is used a culture grown on an agarized wort medium with an immunocytophyte at a concentration of 5.2 × 10 -11 mg / ml DV . The duration of cultivation is 18 days (432 hours), the growth rate is 1.06 cm / day.
EXAMPLE 7 Experiment 5: Grain mycelium was grown under the same conditions as in the control (Example 5), only as a seed material was used a culture grown on an agarized wort media with an immunocytophyte at a concentration of 5.2 × 10 -2 mg / Ml DV. The maximum fouling height is 13 cm. The growing time is 26 days (624 hours), the growth rate is 0.5 cm / day.
EXAMPLE 8 EXPERIMENT 6: Grain mycelium was grown under the same conditions as in the control (Example 5), only as a seed material was used a culture grown on an agarized wort medium with an immunocytophyte at a concentration of 5.2 × 10 -5 mg / Ml DV. The duration of cultivation was 13 days (312 hours), the growth rate was 1.46 cm / day.
EXAMPLE 9 Control 3. Seed is placed on a substrate (straw, sunflower husks, oats). Add chalk. Stirred. The seed material is 3-5% of the volume of the wet substrate. The substrate, together with the seed material, is placed in a standard 401x00 cm polyethylene bag. The height of the substrate in the bag is 80 cm. In a bag with a punch, holes of 15-20 mm in diameter are made, placing them in a staggered order at a distance of 10-15 cm from each other. Substrate humidity is 75%. The growth rate before embryo formation is 2.76 cm / day. Duration of cultivation - 29 days (696 hours). At observance of technological process with 10 kg of a substrate gathering of mushrooms for one rotation makes 1,08 kg.
EXAMPLE 10 Test Seed was grown under the same conditions as in Example 9, only in the presence of an immunocytophyte at a concentration of 5.2 × 10 -5 mg / ml DV. The growth rate before embryo formation was 3.81 cm / day. Duration - 21 days (504 hours). Under the same conditions with 10 kg of substrate, the collection of mushrooms in one rotation is 1.37 kg.
EXAMPLE 11 Test 8. The inoculum was grown under the same conditions as in Example 9 in the presence of an immunocytophyte at a concentration of 5.2 × 10 -11 mg / ml DV. The growth rate before embryo formation was 2.86 cm / day. Duration - 28 days (672 hours). Under the same conditions with 10 kg of substrate, the collection of mushrooms in one rotation is 1.05 kg.
EXAMPLE 12 Test 9. The inoculum was grown under the same conditions as in Example 9 in the presence of an immunocytophyte at a concentration of 5.2 × 10 -2 mg / ml DV. The growth rate before embryo formation is 2.35 cm / day. Duration 34 days (816 hours). Under the same conditions with 10 kg of substrate, the collection of mushrooms in one rotation is 0.72 kg.
The results are shown in the table.
USED BOOKS
1. Patent of the Russian Federation 1697627, A 01 G 1/04, 15.12.92, Bul. 46.
2. The patent of the Russian Federation 1731096, A 01 G 1/04, 07.05.92, Bul. 17.
3. Kulnev AI, Sokolova EA Multipurpose stimulators of protective reactions, growth and development of plants. - Pushchino, 1997. - 18-22 with.
CLAIM
A method for stimulating the growth of a common wolfberry culture, comprising preparing the inoculum on an agarized nutrient medium in the presence of a growth stimulant, characterized in that an immunocytophyte is used as a growth stimulant in a concentration of 5.2 × 10 -5 mg / ml.
print version
Date of publication 11.03.2007gg
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