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INVENTION
Patent of the Russian Federation RU2189728
METHOD FOR GROWING GRAINED MICSELY OF FOOD MUSHROOMS
The name of the inventor: Evdokimova OA; Aksenovskaya V.E .; Usacheva RV; Polish S.V.
The name of the patent holder: Voronezh State Agrarian University. KD Glinka
Address for correspondence: 394087, Voronezh, ul. Michurina, 1, VGAU, Patent Department
Date of commencement of the patent: 2000.10.12
(EN) The invention relates to biotechnology, in particular to methods for preparing cereal mycelium of fungi for subsequent use in fruiting bodies. The invention makes it possible to intensify and reduce the cultivation of commercial mycelium, and to reduce the material and other costs of its preparation. This is achieved by using bags of low pressure polyethylene in a method for growing a grain mycelium, including sterilization of brewed grain with mineral additives, inoculation with the fungal culture and incubation in bags, the sterilization being carried out at a temperature of 120-123 ° C for 1- 2 hours
DESCRIPTION OF THE INVENTION
(EN) The invention relates to biotechnology, in particular to methods for preparing cereal mycelium of fungi for subsequent use in fruiting bodies.
The process of cultivation of commercial mycelium includes the following operations:
- Brewing grain,
- grain sterilization in containers,
- inoculation of grain by the uterine culture,
Incubation of inoculated grain.
An important point in the production of a commercial mycelium is the choice of the container for its cultivation and the way it is closed. In most cases, glass cans (1-3 liters) and milk bottles (0.5-1 liter) are used for this purpose [1, 2]. However, growing mycelium in glass containers has a number of disadvantages:
- the difficulty of sealing the neck of a jar or bottle (usually using cotton-gauze plugs, foil, parchment paper);
- the need to shake the contents of the can (bottle) in the process of growing the crop, which is an additional laborious operation and often with poor closure of the container, leads to infection of the grain;
- the need to re-wrap the mycelium when sold in plastic bags, which may increase the risk of infection of the grain;
- a large glass container fight and the need to wash the cans after each cycle of growing mycelium, and this leads to additional material and labor costs.
A method for growing a mycelium is known, in which the culture after 7-10 days of growing in a glass container is re-introduced into polyethylene bags [3], tightly pack the bags and place them in a refrigerator with a temperature of 2-4 ° C for several days to re-overgrow the grains with culture.
Disadvantages of the method are:
- high probability of contamination of grain by extraneous microflora during re-packing in polyethylene bags;
- additional labor (re-packing);
- Extension of the technological process, t. After re-taking, additional incubation of packages with culture is necessary.
A method for growing a commercial mycelium is also known, in which the brewed grain is sterilized and inoculated in glass containers, and after inoculation, they are re-introduced into polyethylene bags pre-sterilized by the radiation method [1], the culture incubation is carried out in plastic bags in them and the mycelium is sold to the consumer.
This method of growing a mycelium has disadvantages as well as the previous method, and additional costs for the sterilization of packets.
The closest technological essence is the method of growing mycelium in polypropylene bags with bacterial microporous filters. With this method, the brewed grain is sterilized, inoculated and incubated in polypropylene bags. As practice shows, this technology protects the grain from infection as much as possible, but it does not lack any shortcomings:
- often due to poor quality, the polypropylene film becomes rigid after sterilization, which leads to the appearance of microcracks - sources of grain infection;
- the high cost of polypropylene bags with a filter and the inability to reuse them.
The invention solves the problem of intensifying and shortening the time of cultivation of a commercial mycelium, as well as reducing the material and labor costs for its preparation.
This is achieved by using bags of low pressure polyethylene in a method for growing a grain mycelium, including sterilization of brewed grain with mineral additives, inoculation with the fungus culture and incubation in bags, the sterilization being carried out at a temperature of 120-123 ° C for 1- 2 hours.
The claimed method of cultivation of a commercial mycelium suggests carrying out three main stages of obtaining an inoculum: sterilization, inoculation and incubation of grain in bags of low-density polyethylene film (HDPE). The method of growing mycelium is realized as follows:
1) the brewed grain after the addition of mineral additives is packed into polyethylene bags (HDPE) (1 kg each);
2) bags with grain are closed with wooden clothespins and sterilized in an autoclave at a temperature of 120-123 o C 1.0-2.0 hours;
3) after sterilization, the packets are cooled to room temperature and inoculation of the grain by the uterine culture is carried out, observing all asepsis rules;
4) after seeding, the bags are closed with a clothespin, the contents of the bags are well mixed (to distribute the uterine culture evenly throughout the whole grain mass) and incubated in special rooms on the shelves at a temperature of 22-24 o C;
5) after 5-6 days, the packages sprouted with mycelium are shaken to activate the growth of the mycelium. Due to the fact that NVV is a "breathing" film, the mycelium is actively growing;
6) the final readiness of the mycelium is determined visually, the mycelium grows and joins the grains together (10-12 days).
The use of this method of growing a mycelium allows to increase the productivity of the process by 1.4-1.6 times, to reduce material costs (the cost of one package is 10-11 kopecks against 10-30 rubles for a 1-3 l bank and 5 rubles for a polypropylene package) and labor Costs (there are no inherent operations in other ways: re-taking of the cultivated mycelium and washing dishes).
USED BOOKS
1. The journal "School of Mushrooming" 3, 2000, p. 20-22 is a prototype.
2. Dudka IA, Bisko N.A. Biology and cultivation of edible fungi of the genus Veshenka. - Kiev, Naukova Dumka, 1987.
3. The invention according to the patent of the Russian Federation 2101914 "A way of preparation of a cereal mycelium of the higher fungi". A 01 G 1/04, 20.01.98. Bul. 2.1
CLAIM
A method for growing a cereal mycelia of food fungi, comprising sterilizing the brewed grain with mineral additives, inoculating the fungus culture and incubating in bags, characterized in that low-density polyethylene bags are used, the sterilization being carried out at a temperature of 120-123 ° C for 1-2 hours .
print version
Date of publication 11.03.2007gg
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