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INVENTION
Patent of the Russian Federation RU2013931
NUTRIENT ENVIRONMENT FOR GROWING THE MICRELINE OF EDITED MUSHROOMS
The name of the inventor: Vasilieva AN
Name of patent holder: Siberian Research Institute of Peat
Address for correspondence:
Date of commencement of the patent: 1991.06.03
Use: agriculture, in particular with the artificial breeding of edible fungi. SUMMARY OF THE INVENTION: The nutrient medium contains (in wt.%) 12.0 to 24.0 grains of wheat, 29.1 to 60.4 of a natural zeolite, 1.0 to 2.0 gypsum, 0.4 to 0.8 chalk and Water.
DESCRIPTION OF THE INVENTION
The invention relates to agriculture, in particular to the artificial cultivation of edible fungi, and can be used in farms for the cultivation of edible fungi, in the production of seeded mycelium.
A nutrient medium is known for growing a mycelium of edible fungi (1) containing nutrient, claydite, gypsum, chalk, water, and as a nutrient component it contains dried and powdered larvae of synanthropic flies and starch.
The disadvantage of this nutrient medium is the exoticity (deficiency) of the introduced additive in the form of larvae of synanthropic flies, and the consumption of food - starch.
The closest to the claimed invention is a nutrient medium for growing the mycelium of edible fungi (2) containing wheat, rye, oats, millet, corn and other cereals as a nutritional component. Grain in a known nutrient medium is prepared as follows: to 10 kg add 15 liters of water and cook for 15-30 minutes on low heat. The broth is drained through a sieve, the grain is scattered on a clean surface with a layer of 2-3 cm for drying. Then 30 g of chalk and 12 g of gypsum are added to the grain to regulate the acidity and improve the structure.
The aforementioned nutrient medium has the following disadvantages: poor quality of the medium due to coalescence of grains and deterioration of physical properties, as well as high cost of the nutrient medium due to the presence of a food product-grain in it.
The object of the invention is to improve the quality of the nutrient medium by improving the physical properties, and to reduce its cost due to a partial replacement of the food product.
The aim is achieved in that, in a nutrient medium for the cultivation of a mycelium of edible fungi, comprising a carrier and a nutritional component, gypsum, chalk, according to the claimed invention, it contains, as a carrier, a natural zeolite, and as a nutrient component, wheat grain in the following ratio, %: Dry grain of wheat 12,00-24,00 Zeolite 29,10-60,40 Gypsum 1,00-2,00 Chalk 0,40-0,80 Water Other.
In the prior art, there are no features that are similar to the features of the claimed invention.
EXAMPLE. Seven nutrient media for growing oyster mushrooms (Pl. Floridae) are tested, which include natural zeolites (clinoptilolites) and wheat grains as main components, as well as gypsum, chalk, and water.
The composition of the tested nutrient media is shown in Table. 1.
To prepare a nutrient medium, the grain is steamed, dried, mixed with gypsum and chalk by a known technology. Zeolite is mixed with the grain in a certain weight ratio. The prepared culture medium is filled with containers for growing the planting mycelium of edible fungi and sterilized in an autoclave at 2.0 atm for 2 hours.
The medium is inoculated with an intermediate culture (2-3 grains per 0.1 kg of medium) and incubated for 15 days.
5-7 days after inoculation, shaking of the media in the vessels is carried out to accelerate their overgrowing with fungal mycelium.
Observe the nature and rate of growth of the mycelium on the control and experimental nutrient media.
The results of observations of the growth of mycelium are given in Table. 2.
Mycelium oyster mushrooms, grown on experimental nutrient media, by all indices are not inferior to cereals, and over fecundity exceed it.
In all variants of the tested media, there was no extraneous microflora. However, the introduction of the zeolite in an amount exceeding 60 wt. %, Worsened, reduced the intensity of growth of the mycelium. When the amount of zeolite is less than 29% by weight, a poor friability of the mycelium is noted. Thus, the optimum amount of zeolite introduced, necessary to improve the physical properties of the mycelium and save grain, lies in the range 29.1-60.4 wt%.
The grown mycelium is used as a planting material when growing oyster mushrooms on straw. The harvest of oyster mushroom obtained using cultivated mycelium is shown in Table. 3.
The growth of the mycelium of oyster mushrooms in the substrate from straw on microblocks (weighing 1.5 kg) in the control and in the fifth variant did not differ in speed and intensity.
According to the experimental data obtained at the control and experimental sites, the yield of oyster mushrooms is practically the same.
The obtained results showed that the tested nutrient mediums, not containing only deficit wheat grain, allow the planting material (mycelium) of oyster mushrooms to grow in quality, not inferior to the mycelium obtained on nutrient media containing grain.
The claimed nutrient medium has the following advantages in comparison with the prototype: the best physical properties due to the introduction of zeolite into its composition; Cheapness due to the partial replacement of grain with zeolite, while maintaining all the previous properties of the prototype.
CLAIM
NUTRIENT ENVIRONMENT FOR GROWING THE MICRELINE OF EDITED MUSHROOMS, containing wheat grain, gypsum, chalk and water, characterized in that it additionally contains natural zeolite at the following ratio of components, mass%:
Wheat grain 12.0 - 24.0
Natural zeolite 29.1 - 60.4
Gypsum 1.0 - 2.0
Chalk 0.4-0.8
Water Other
print version
Date of publication 11.03.2007gg
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