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INVENTION
Patent of the Russian Federation RU2104534
METHOD OF DIAGNOSTICS OF HELMINTOSE
The name of the inventor: Kolosovsky Ernest Dmitrievich; Korovin Konstantin Fedorovich; Queen Elena Mikhailovna
The name of the patent holder: Kolosovsky Ernest Dmitrievich; Korovin Konstantin Fedorovich; Queen Elena Mikhailovna
Address for correspondence:
Date of commencement of the patent: 1996.05.08
The proposed method for the determination of ascarids in the human intestine by the method of high-performance liquid chromatography by the products of helminth metabolism in urine is accurate and allows to conduct a study at any convenient time, and to diagnose helminthiasis at an early stage of the disease (larval), when eggs of helminths in the stool are absent.
DESCRIPTION OF THE INVENTION
The invention relates to medicine, in particular to laboratory methods of investigation.
Aksaridoz, chronic helminthiasis are found in various regions of Russia in approximately 7-11% of children.
Aksaridoz is characterized by the presence of an allergic syndrome in the early phase of the disease and abdominal - in the late phase. Exciter of ascaridosis - Ascaris Lumbricoides - large nematodes spindle-shaped. The length of the male is 15-20 cm, the female is much larger than 25-40 cm. The female of the roundworm lays fertilized and unfertilized eggs daily around 200,000 pieces. When invasive eggs enter the small intestine, larvae develop in the small intestine and penetrate into the venous vessels of the mucous membrane and enter the portal vein system, into the right half of the heart, and then into the capillary network of the lungs. During the migration, the larvae grow to 2-2.6 mm, then actively enter the lumen of the alveoli, ascend to the bronchioles, bronchi, trachea and pharynx. Once in the mouth, the larvae, along with saliva, enter the small intestine, where adult individuals develop. Migration of larvae lasts 2-3 weeks. The entire cycle of development of ascarids from the moment of human infection to the appearance of new generation in feces is 2.5-3 months. Life expectancy of ascarids in the human body is 9-12 months, the period of ovulatory activity of the female is about 7 months, oviposition stops within the last 2 months of parasitization (Yarovoy PI, Zubchuk MP, Tokmalayev AV Handbook of Helminthology Chisinau, 1984).
Known method for determining helminth eggs, incl. Ascaris by flotation in excrements (Kalantaryan EV Medical parasitology, 1938. Predtechensky VE, Borovskaya VM, Margolina LG Laboratory methods of research, Moscow, 1950).
Disadvantages of this method are:
1) In the larval stage, eggs are not deposited; 2) oviposition stops in the last two months of the existence of helminths; 3) the male eggs do not lay eggs; 4) in the method of studying feces, morning feces are required for eggs of worms, but children who have ascarids often have stools in the evening; 5) existing methods of research of ascaris eggs in feces give a low percentage of detection, because there may not be helminth eggs in the test portion, despite their presence in the intestine.
The aim of the invention is to increase the accuracy and reliability of the method for diagnosing helminthiosis in the human intestine and to identify an early stage (larval) disease when helminth eggs are not present in the stool.
To this end, we use the method of high-performance liquid chromatography (HPLC), with which we determine the presence of helminths in the content of products of their metabolism in urine, including:
1. Formic acid - formik acid (C1);
2. Acetic acid - acetic a. (C2);
3. Propionic acid - propionic a. (C3);
4. Butyric acid - butiric a. (C4);
5. Valeric acid - valeric a. (C5);
6. Caproic acid - caproic a. (C6);
7. Caprylic acid - caprilic a. (C7);
Capric acid - capric a. (C10);
9. Lauric acid - lauric a. (C12);
10. Myristic acid is a miristic a. (C14);
11. Palmitic acid - palmitic a. (C16);
12. Stearic acid - stearic a. (C18);
13. Lactic acid - latic a.
14. Alpha-methylbutyric acid-alpha-methylbutiric a.
15. Alpha-methylcrotonic acid-alpha-methylcrotonic a.
16. Alpha-methylvaleric acid - alpha-methylvaleric a.
We studied urine in 6 children, in which eggs of the ascarid worm in the feces were found by the usual method.
It turned out that the level of formic, caproic and propionic acids was increased in comparison with that of the children of the control group (in which helminths were not found), which makes it possible to conclude that the proposed method is advisable.
It has been established that helminths can negatively influence the state of host resistance to bacteria, viruses, protozoa; Their presence in the patient's body often delays the period of bacteriocarrier, and when vaccinations are carried out, it lowers the level of immunity immunity. Ascarids adversely affect the course of infectious diseases (bacterial dysentery, typhoid fever, salmonellosis), childhood infections and skin diseases.
The presence of ascarids in the human intestine, larvae in the lungs (pulmonary form) cause various lesions of the gastrointestinal tract and skin, accompanied by abdominal pain, weakness and other numerous symptoms of digestive organs, which complicates the diagnosis.
Therefore, the technique developed by us will allow us to conduct a wide survey of children's groups for the presence of ascaridosis.
The advantage of this method is that the urine for research in children and adults can be obtained at any time, it can be stored in the refrigerator at a temperature of 4-6 degrees and investigate HPLC with a sufficient degree of certainty, and that worms are detected at an early Stage of the disease (larval), when eggs of helminths in the feces are absent.
CLAIM
The method of diagnostics of helminthiosis, characterized in that urine is used to study the metabolic products of ascarids, for example, formic, caproic and propionic acids, by high-performance liquid chromatography and to ascertain the presence of ascarids to increase their content.
print version
Date of publication 06.01.2007gg
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