Navigation: =>

Home / Patent catalog / Catalog section / Back /

GASTROENTEROLOGY

INVENTION
Patent of the Russian Federation RU2241997

METHOD FOR DETERMINATION OF OXIDATIVE-RESTORATION POTENTIAL OF STOMACH OF THE STOMACH AND TWELFTH DIAGNOSTIC

METHOD FOR DETERMINATION OF OXIDATIVE-RESTORATION POTENTIAL OF STOMACH OF THE STOMACH AND TWELFTH DIAGNOSTIC

The name of the inventor: Evseev MA (RU); Gostishchev V.K. (RU); Nikolaev SG (RU)
The name of the patent holder: Evseev Maxim Aleksandrovich (RU)
Address for correspondence: 125373 , Moscow, Vasily Petushkov, 21-2-152, MAAvseevu
Date of commencement of the patent: 2003.11.18

(EN) The invention relates to the field of medicine. The method is characterized in that an active needle platinum electrode with a diameter of 0.6 mm and a length of 3 mm is inserted through the fibroadastoduodenoscope, which is implanted into the site of the wall of the organ under examination perpendicular to the mucosal plane to a depth of 2 mm and the value of the redox potential is determined from the difference in potentials that have arisen Between the active electrode and the reference electrode in not less than 1 min. The invention makes it possible to more accurately determine the value of the oxidation-reduction potential.

DESCRIPTION OF THE INVENTION

(EN) The invention relates to medicine, in particular to methods for measuring the oxidation-reduction potential (Eh) of the wall of the stomach and duodenum.

There is a known method for determining the pO 2 of the gastric mucosa (Zatevakhin II et al., In the book "New technologies in the treatment of ulcerative gastroduodenal bleeding." - M., 2001).

To determine pO 2 in the gastric mucosa, a polarographic study was carried out using the original endoscopic technique: an active platinum electrode of contact type with a conductor was conducted through the biopsy channel of the fibroadastoduodenoscope; a close contact of the active electrode with the gastric mucosa was carried out; A calomel electrode was used as the reference electrode, and a polarograph was used as the recording device.

The method has the following drawbacks:

With this method of detection, the electrode and hydrochloric acid of the gastric juice inevitably come into contact and, therefore, the error in measuring pO 2 by the clearance of H + .

Technical difficulties in retaining a close contact of the electrode with a given region of the mucosa with the mobility of the wall of the stomach (peristalsis, breathing, insufflation of air) leads to a displacement of the electrode and also to an error in the measurement.

The need to calibrate the active electrode before each measurement; Indirect (polarography) measurement of the pO 2 value involves distortion of the true values.

An object of the present invention is to examine Eh in the wall of the stomach and duodenum.

The problem is solved by the fact that a method for determining the oxidation-reduction potential of the wall of the stomach and duodenum is proposed, which consists in introducing an active needle-shaped platinum electrode with a diameter of 0.6 mm and a length of 3 mm through the fibroadastoduodenoscope, which is implanted into the wall portion of the organ under examination perpendicular to Mucosal plane to a depth of 2 mm and the value of the oxidation-reduction potential are determined from the difference in potentials between the active electrode and the reference electrode in not less than 1 minute.

The study consists in recording the oxidation-reduction potential (Eh) arising between the measuring electrode implanted in the submucosal layer of the wall of the stomach (duodenum) and the reference electrode.

The measuring electrode is a platinum needle electrode with a diameter of 0.6 mm and a length of 3 mm. The measuring electrode is connected to the recording device by means of a conductor with a length of 2000 mm and a diameter of 1 mm. The conductor, the place of connection of the active electrode with the conductor and the closest to the conductor of 1 mm of the active electrode are covered with a layer of insulator (polyvinylchloride). The total diameter of the conductor and insulator should not exceed 2 mm.

The reference electrode is silver chloride, standard, factory-made. The reference electrode is placed in the left axillary cavity of the patient, the reference electrode and the skin are contacted by means of a gauze pad impregnated with 0.9% sodium chloride solution.

The recording device is a voltmeter with a measurement range of at least -300 ... + 300 mV, with a resolution of 1.

The potential of the measuring electrode relative to the reference electrode is checked in control solutions in accordance with GOST 8.450 at a temperature of + 25 ° С + 1 ° С.

Fibrogastroduodenoscope is inserted into the lumen of the stomach (duodenum), the end part of the fibrogastroduodenoscope is set opposite to the investigated site of the organ wall at a distance of 5 mm from the latter.

Through the working channel of the fibrogastroduodenoscope into the lumen of the stomach (duodenum), an active electrode with a conductor is introduced.

The active electrode is implanted into the examined area of ​​the stomach wall (duodenum) perpendicular to the plane of the mucous membrane to a depth of 2 mm until the insulator fully contacts the mucosa.

The recording device is switched to the mode of determining the potential. The true value of the oxidation-reduction potential is the value recorded at least 1 minute after the start of the measurement.

The proposed method makes it possible to establish the ratio of the activity of oxidants and antioxidants to tissues according to formula

Eh = Eo + klg [Ox] / [Red],

Where E0 is the stationary oxidation-reduction potential for a given temperature,

[Red] is the concentration of reducing agents in the tissue,

[Ox] is the concentration of oxidants in the tissue.

For Eh Eo in the tissue prevail are the processes of recovery,

At Eh> Eo - oxidation processes.

The ratio [Ox] / [Red] and, consequently, Eh reflects the activity of the processes of peroxidation and the consistency of the antioxidant system of this tissue under the given conditions.

[Oх] is noted in the presence of tissue ischemia or with reperfusion syndrome with the development of tissue necrosis.

Experimental measurements were made:

In rats of the white line, Eh was measured in the thigh muscles (Eh = + 10 mV). The ischemia of the tissue was simulated by temporary aortic compression for 15 min, while Eh Up to +60 mV. With the resumption of blood flow Eh (up to + 20-15 mV (histologically - acute muscular dystrophy) .For aortic compression more than 15 min Eh > 90 mV (histologically - tissue necrosis).

The method was approved on the basis of Moscow hospitals.

In volunteers in the absence of pathology of the stomach and duodenum Eh = + 15 mV. In conditions of acute tissue bleeding Eh Up to + 60-70 mV. Subsequently, acute erosive and ulcerative lesions of the wall of the stomach or duodenum, which was predicted by Eh.

Thus, a change in the Eh values ​​as compared to the Eo norm serves as a criterion for determining tissue necrosis.

CLAIM

A method for determining the oxidation-reduction potential of the wall of the stomach and duodenum, characterized in that an active needle-shaped platinum electrode with a diameter of 0.6 mm and a length of 3 mm is inserted through the fibrogastroduodenoscope, which is implanted into the wall portion of the organ under examination perpendicular to the mucosal plane to a depth of 2 mm and The value of the oxidation-reduction potential is determined from the difference in potentials between the active electrode and the reference electrode in not less than 1 minute.

print version
Date of publication 06.01.2007gg