Start of section
Production, amateur
Radio amateurs
Aircraft model, rocket-model
Useful, entertaining 		Stealth Master
Electronics
Physics
Technologies
Inventions 		Secrets of the cosmos
Secrets of the Earth
Secrets of the Ocean
Tricks
Map of section
Start
Forum
Use of the site materials is allowed subject to the link (for websites - hyperlinks)

Navigation: =>

Home / Patent catalog / Catalog section / Back /

DERMATOVENEREOLOGY
INVENTION
Patent of the Russian Federation RU2084202

METHOD FOR TREATMENT OF MANIFESTATIONS OF PAPILLOMOVIRUS INFECTION ON SKIN AND MISSILE SHELLS

METHOD FOR TREATMENT OF MANUFACTURES OF PAPILLOMOVIRUS INFECTION
ON SKIN AND MISSILE SHELLS

The name of the inventor: Bogatyreva II; Denisov LA; Volkova EV
The name of the patent holder: Central Dermatovenerologic Research Institute of the Ministry of Health and the Ministry of Health of the Russian Federation
Address for correspondence:
Date of commencement of the patent: 1995.09.22

Use: The invention relates to the field of medicine, in particular to methods for treating manifestations of papillomavirus infection on the skin and mucous membranes. SUMMARY OF THE INVENTION: The method includes cryopreservation, which allows the boundaries of the lesion to be clearly defined, and the subsequent treatment by an autonomous cryoapplicator. After the cryoexposure, an ointment with interferon containing dimethyl sulfoxide is immediately applied, which ensures that the interferon is brought to the basal and parabasal layers of the epithelium.

DESCRIPTION OF THE INVENTION

The invention relates to the field of medicine and can be used to treat manifestations of papillomavirus infection (viral warts, condylomas) on the skin and mucous membranes.

Known is a method for treating manifestations of papillomavirus infection in the mucous membrane of the genitals in women, consisting in cryopreservation with a wooden probe with a cotton swab before whitening, and then applying an ointment on a vaseline base containing interferon.

However, cryoexposure by a wooden probe with a cotton swab does not provide for a dosed exposure to an adequate temperature for the necessary cryopreservation of lesions caused by papillomavirus; To maintain the desired temperature, the tampon is constantly immersed in a vessel with liquid nitrogen, the procedure is thus interrupted, and the effect of cryovation is slowed by self-heating of the tissues.

The use of leukocyte interferon in a vaseline-based ointment is not effective enough, since the active ingredient can not be stably delivered to the basal and parabasal layers of the epithelium where replication of the papillomavirus belonging to the class of DNA viruses takes place. In this regard, the therapeutic process can be difficult, and it is possible to increase the area of ​​the lesion, as cryotestruction of epithelial cells occurs with insufficient stimulation of immunocompetent cellular elements responsible for the destruction and phagocytosis of viral particles. Given the oncogenic potential of some varieties of human papillomavirus associated with the genital area, especially in women, inadequate curative effects can lead to malignancy.

The invention is aimed at improving the treatment of manifestations of papillomavirus infection on the skin and mucous membranes by providing a dosed adequate low-temperature exposure with a preliminary clear definition of the area of ​​the lesion with further use of an ointment having a composition providing delivery of interferon to the basal and parabasal layers of the epithelium.

The method of treatment is that, in the presence of manifestations of papillomavirus infection on the skin and mucous membranes, a cryospiration is produced in the course of 10-30 s, at which the borders of the lesion are clearly revealed due to the characteristic papillary surface of the lesions, which at the same time becomes white. Cryospiration is also a curative procedure. In the presence of lesions on the mucous membranes cryospiration reveals hidden forms that are not determined visually. After determining the boundaries, the lesion focus is treated with an autonomous cryoapiliaryator at a temperature from -160 to -180 o C with the capture of adjacent tissues 3-4 mm within 15-60 seconds, depending on the localization of the lesion. Cryodestruction is expedient to produce twice after spontaneous self-heating of tissues.

Immediately after the cryoprocedure, a thin layer of ointment containing interferons, obtained mainly by genetic engineering, is applied. Interferons alpha, gamma, or a mixture thereof can be used. The ointment is produced on a cosmetic emulsion base of the "water / oil" type, into which dimethylsulfoxide is added in an amount of 20-50% by weight. The presence of dimethylsulfoxide ensures the delivery of interferon to the basal and parabasal layers of the epithelium; The conductive effect is enhanced by the use of a cosmetic emulsion base.

For the preparation of the ointment, a cosmetic emulsion base containing the following components (by weight) can be used:

  • Wax base 5,0
  • Lanolin anhydrous 6.0
  • Confectionary fat 5,0
  • Ceresin 2.0
  • Paraffin 1.0
  • Vegetable oil 30,0
  • Oil vaseline 9,0
  • Synthetic alcohols 1,5
  • Pental 6.0
  • Purified water and preservatives

In the presence of papillomavirus infection in the urogenital tract, candles based on cocoa butter, including interferons, are additionally used.

When expressed manifestations of papillomavirus infection on the skin (vulgar, plantar warts, viral pseudoepitheliomatous hyperplasia) before cryoexposure, microwave exposure is applied for 60-120 s. Microwave effect can be performed with the help of devices used in medical practice, such as "Thermik", "Luch-1", "Luch-2"

In the presence of large lesions on the mucous membranes (giant condylomas) or manifestations of papillomavirus infection, previously treated by other methods causing cicatricial changes, before cryo-exposure, the lesion is subjected to ultrasound exposure for 40-60 seconds. Ultrasonic exposure can be achieved with the ultrasound therapy device UZT-104.

The method is illustrated by the following clinical examples (based on the department of dermatology of the Central Dermatovenerologic Institute).

Example 1 . Patient B. is 20 years old, with a plantar wart in the foot area measuring 1 cm in diameter. After removal of the hornfels from the lesion, it was subjected to microwave exposure for 60 s with the Luch-1 apparatus, then immediately by cryopreshing within 10 seconds of determining the border of the lesion. Next, a nozzle was chosen for an autonomous cryoapplicator, corresponding to the size of the lesion focus. Cryoprotection with a cryoapplicator was performed twice for 60 seconds with an interval for self-heating of the tissue. Then an ointment was applied for external treatment and the lesion was covered with adhesive tape. 400 g of ointment prepared on a cosmetic basis contain the following components: recombinant human interferon alfa (Reaferon), produced by VECTOR, Novosibirsk 1 000 000 ME, recombinant human interferon gamma (Degaferon), produced by SPA Biosintez, Moscow region . 100,000 IU, dimethylsulfoxide 100.0 g

Recovery occurred after three procedures once a week with a preliminary cleansing of the lesion from necrotic masses.

Example 2 . Patient V. 19 years old, with genital warts entering the vagina and damaging the walls of the vagina. Cryospiration was performed within 20 seconds, the boundaries of the lesion were established. Then the cryoapplicator performed a cryodestruction of lesions located on the outer genitalia 2 times for 20 seconds. After that, the ointment on a cosmetic basis, containing 400 g of ointment: recombinant human interferon gamma (Degaderon) 1 000 000 ME, dimethyl sulfoxide 200 g was applied to the external part of the genitalia. A candle made on the basis of cocoa butter and containing interferon gamma was introduced into the vaginal area In the amount of 10 000 ME. At home, the patient twice daily applied ointment, suppositories were applied daily for 10 days. Recovery came after 4 sessions of cryotherapy with subsequent application of the ointment. The sessions were held twice a week.

Example 3 . Patient B. 25 years old, with condylomata on the mucous membrane of the genitals and cicatricial changes after preliminary treatment with destructive agents (selezole). The lesion was exposed to ultrasound for 60 s with ultrasound therapy device UZT-104 with an intensity of 10 W / cm 2 . Then, with the help of cryoprecipitation within 15 s, the boundaries of the lesion were determined. Further, a cryodestruction was performed with the help of an autonomous cryoapplicator twice for 30 seconds with an interval for spontaneous tissue self-heating. After cryoexposure, a cosmetic-based ointment containing 400 g of ointment: recombinant human interferon alfa (Reaferon) 10 000 000 IU, recombinant human interferon gamma (Gammaderon) 1 000 000 IU, dimethyl sulfoxide 40.0 g. Application of the ointment recovery occurred after 6 sessions of cryoexposure 2 times a week.

CLAIM

1. A method for the treatment of manifestations of papillomavirus infection on the skin and mucous membranes, comprising cryo-exposure followed by application of an ointment containing interferon, characterized in that the cryoprotection is carried out by cryopreservation for 10 30 seconds, followed by treatment with an autonomous cryoapplicator, further comprising dimethylsulfoxide .

2. The method of claim 1, characterized in that, in the lesion of the urogenital tract, the addition of suppositories containing interferon is additionally used.

3. A method according to claim 1, characterized in that, before cryopreservation, the lesion is subjected to microwave exposure.

4. A method according to claim 1, characterized in that, before cryopreservation, the lesion is subjected to ultrasonic action.

print version
Date of publication 01.04.2007гг