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INVENTION
Patent of the Russian Federation RU2285529
METHOD OF TREATMENT AIDS
The name of the inventor: Lobzin Yuri Vladimirovich (RU); Alexandrov Viktor Nikolaevich (RU); Makeev Boris Lavrovich (RU)
The name of the patent holder: Military Medical Academy. CM. Kirov (RU); Lobzin Yuri Vladimirovich (RU); Alexandrov Viktor Nikolaevich (RU); Makeev Boris Lavrovich (RU)
Address for correspondence: 194044, St. Petersburg, ul. Acad. Lebedeva, 6, Military Medical Academy. CM. Kirov, NIO, patent expert
Date of commencement of the patent: 2004.07.16
The invention relates to medicine, to infectious diseases and can be used to treat AIDS. Step-by-step treatment is carried out, in which, at the first stage, the inhibitor and activator of viral replication are alternately introduced; At the second stage, hemosorption is carried out, the third is carried out with the rehabilitation of the immune system using blood enriched with stem cells and specific immunostimulation; Hemosorption is carried out using columns, each of which contains a matrix with agarose immobilized ligands, respectively: the first one is specific antiviral antibodies, the second is the CD4 receptors, the third is the gamma-interferon receptors, the fourth is the receptors for the Fc fragment of the immunoglobulin G, the fifth is antimicrobial proteins of animal origin, and the blood enriched with stem cells is administered intravenously. This invention contributes to the maximum sorption of HIV and its elimination, to the restriction of iatrogenic provocation of immunosuppression, which, in turn, helps to reduce the risk of persistence of the virus in the patient's body.
DESCRIPTION OF THE INVENTION
The invention relates to the field of medicine, in particular to infectious diseases, and can be used to treat HIV infection.
A method for treating HIV infection, including transplanting a patient with an immunogenic cell suspension, is known, the treatment being carried out on the basis of the principles of biological informatics, in which the patient is pre-extracorporeally purified by plasmosorption on a FAS brand sorbent that is covalently bound to antibodies produced using hemoculture from And covalently linked to a glucoprotein isolated from the human placenta, followed by a course of immunostimulation with an autovaccine made on the basis of inactivated hemoculture from the patient, followed by transplantation into the bone marrow of cord blood taken from the umbilical cord cut from the placenta. (Patent of the Russian Federation No. 2146930, IPC 6 A 61 K 35/16, A 61 M 1/36).
The disadvantage of this method is the possibility of prolonged persistence of the virus in the patient's body.
The aim of the invention is to reduce the risk of the persistence of the virus in the patient's body.
The object is achieved by the fact that the method comprises a stepwise treatment in which, in a first step, an inhibitor and a replication activator are alternately administered; Hemosorption is carried out in the second stage, the third is carried out with the rehabilitation of the immune system using blood enriched with stem cells and specific immunostimulation, with hemosorption being carried out using columns, each containing a matrix with agarose immobilized ligands, respectively, the first specific antiviral antibodies, The second - CD4 receptors, the third - receptors for gamma-interferon, the fourth - the receptors for the Fc-fragment immunoglobulin - G, the fifth - antimicrobial proteins of animal origin, and the enriched blood stem cells are intravenously administered.
METHOD REALIZES AS FOLLOWED
At the first stage, the reverse transcriptase inhibitor and polysaccharides are alternately administered; In a second step, hemosorption is carried out using columns of four matrices with immobilized ligands involved in the process of introducing the virus into the cells; The columns immobilize antibodies against the viruses of the patient: - recombinant CD-4 receptor; - receptor for gamma interferon; - receptor to the Fc-fragment of immunoglobulins of antimicrobial proteins of animal origin. In the third stage, the immune system is rehabilitated by intravenous cord blood injection; At the final fourth stage, immunostimulation is carried out by introducing a specific autovaccine.
The positive results of the treatment received by the author of the invention, accepted by us as a prototype (patent No. 2146930 under application No. 98107018/14 of 10.04.98), are strengthened primarily by new elements: a strict sequence of stages constructed taking into account the pathogenesis of HIV infection.
The first stage allows to ensure the synchronization of the phases of virus replication and thus the formation of conditions favorable for the elimination of HIV virions, which concentrate in the blood plasma.
The second stage allows to eliminate viruses from the blood and fix them on the matrices through ligand-receptor interactions.
As solid-phase carrier, coarse agarose 4B (0.4 to 1 mm in diameter) is used, which has good drainage properties for the blood elements.
Activation of the agarose is carried out with cyanogen bromide. Stages of immobilization of ligands, blocking of remaining active valences and washing of sorbents are carried out according to the recommendation of Y.Turkova (1980).
As active ligands, rabbit affinity purified antibodies against the viral antigens of a particular patient were used. Fragments of immunoglobulins were obtained from hyperimmune sera by affinity chromotography on columns with solid phase protein A. Stafylococcus aureus. The specific capacity of each sorbent was determined by chromatography and electrophoresis methods.
The third stage provides restoration of the regulatory and effector compartments of the immune system through the proliferation and differentiation of transplanted lymphoid stem-forming cells.
The fourth stage provides a specific (for a given patient) antiviral stimulation of the immune system with the accumulation of cytostatic lymphocytes and antibodies.
CLAIM
A method of treating AIDS, comprising the stepwise treatment, in which, in a first step, the inhibitor and activator of viral replication are alternately administered; Hemosorption is carried out in the second stage, the immune system is rehabilitated with the use of blood enriched with stem cells and a specific immunostimulation, characterized in that hemosorption is carried out using columns, each containing a matrix with agarose immobilized ligands, respectively, the first - specific Antiviral antibodies, the second - CD4 receptors, the third - receptors for gamma-interferon, the fourth - receptors for Fc-fragment of immunoglobulin G, the fifth - antimicrobial proteins of animal origin, and the enriched blood stem cells are intravenously administered.
print version
Date of publication 09.01.2007гг
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