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INVENTION
Russian Federation Patent RU2097427
Method for inactivating the AIDS virus
Name of the inventor: Fedchenko Peter; Tuev Vladimir Vladimirovich
The name of the patentee: Fedchenko Peter; Tuev Vladimir Vladimirovich
Address for correspondence:
Starting date of the patent: 1996.02.22
Usage: AIDS virus inactivation method relates to virology, and in particular to means of neutralizing the AIDS virus radiation. The inventive viral material is irradiated with a combined light corresponding to electronic transitions magnesium atoms 516.7; 517.2; 518.2 nm and 636.2 nm zinc atoms within 30 - 60 min, the virus causing AIDS, substantial modifications in nucleic acids and in the reverse transcriptase enzyme, which leads to a virus inactivation.
DESCRIPTION OF THE INVENTION
The invention relates to virology, and in particular to means of neutralizing the AIDS virus radiation.
A method is known AIDS virus inactivation by irradiation with the light at a wavelength of 636.2 nm for 30 minutes 60 [1]
The disadvantage of this method is the low inactivation of AIDS virus.
The object of the invention is a large virus inactivation of the enzyme system.
It is known that as the AIDS virus genetic material is RNA molecule comprising, as is known, five magnesium atoms. RNA molecule would serve only in the event of its reliable protection against the disturbing effects of light on the electronic transitions of magnesium atom.
It is also known that the process of reverse transcription in the AIDS virus, like other retroviruses, by using the enzyme reverse transcriptase (reverse transcriptase), which operates with zinc atoms. The success of this process will flow only when the electron shell of the atom and zinc (as a magnesium atom) will be protected from light corresponding to a wavelength of 636.2 nm. In this connection, it may be assumed that under the influence of light on the AIDS virus in the electronic transitions of magnesium atoms (516.7; 517.2; 518.2 nm), and electronic transitions of atoms of zinc (636.2 nm) may be affected by a nucleic acid, so and the enzyme reverse transcriptase, which is significantly lower virus activity.
A special experiment was carried out in this regard, is as follows.
In the model cell lymphoblastoid cell lines CEM and MT-4 infected with human immunodeficiency virus (HIV) is the strain HIV-1 / B RU (acute infection), studied the possibility of radiation virus neutralization.
Materials and methods. Cells. MT-4 cells were grown in suspension in PPMI 1640 medium with 15% fetal calf serum, 100 micrograms / ml gentamycin in a concentration of 500,000 to 300,000 cells per 1 ml.
Virus. The strain of HIV-1 / B RU received from the museum of the Institute of Virology RAMS strains.
Evaluation of antiviral action performed by monochromatic light sintsitieobrazovaniya virus-induced inhibition, but also the accumulation of viral antigen in the culture fluid detected by ELISA.
Results and discussion. Before infecting sensitive cells with HIV-1 viral material is irradiated in the visible spectrum over magnesium lines (516.7; 517.2; 518.2 nm), and zinc lines (636.2 nm) alone and in combination, with the net power of light less than 0.1 watts with an exposure of 30 minutes and 60 minutes.
After receiving the monochromatic light of the virus sample material introduced into the wells of cell culture. As a positive control, non-irradiated virus (multiplicity of infection TCD 0,1 lg / 50 cells) uninfected cells were negative.
Infected cultures were incubated for 4 to 7 days in an atmosphere with 5% CO 2 and 98% humidity at 37 o. Experimental results are presented in the table.
The data show that in the case of the AIDS virus combined irradiation light corresponding to electron transitions of zinc and magnesium atoms, virus-induced syncytia formation is reduced by 57.7 and 30.8% respectively. It was noted a marked decrease in the accumulation of viral antigen in the culture fluid of cells infected with virus.
The combined use of monochromatic light corresponding to electronic transitions in atoms of magnesium and zinc, reducing the number of additive caused the virus-induced syncytia and the level of viral antigen. The use of radiation in the visible spectrum, obviously does not affect the outer membranes of the drug with unique biological properties.
Comparative Experiment AIDS virus exposure was carried out as follows.
First, viral material alternately irradiated with light at wavelengths (516.7; 517.2; 518.2 nm), and then the same virus material with rupture time of 15 min 20 irradiated with light with a wavelength of 636.2 nm. The result of this experiment it was found that virus inactivation occurs, but not higher than if only one we shone any light.
And only when the material was irradiated viral combined light simultaneously (without any time gap), its inactivation increased significantly.
BIBLIOGRAPHY
Fedchenko PP spout DN Simonov VS Konstantinov NV The process of inactivation of the AIDS virus. Patent N 2031945 from 03.27.1995.
CLAIM
AIDS virus inactivation method comprising exposing light with a wavelength of 636.2 nm for 30 minutes 60, characterized in that the light exposure is carried out in combination with a light flux with wavelength 516.7 nm, 517.2 nm, 518.2 nm .
print version
Publication date 06.01.2007gg
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