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INVENTION
Russian Federation Patent RU2019186

METHOD inhibition of HIV infection

METHOD inhibition of HIV infection

Name of the inventor: Bykovskii AF .; Miller GG .; Pokidysheva LN .; Titov IV .; Artyukov AA .; Popov AM .; Prozorovsky SV .; Loenko YN .; Joseph Hess [US]; Elyakov G.B .; Stonik VA .; Isakov VV
The name of the patentee: Scientific Research Institute of Epidemiology and Microbiology im.N.F.Gamalei; Pacific Institute of Bioorganic Chemistry
Address for correspondence:
Starting date of the patent: 1991.02.28

Use: in medicine, in particular for the prevention and treatment of AIDS and AIDS-related diseases. The inventive method utilizes a low molecular weight sulfated polysaccharide mol. at. less than 20,000 Daltons with fucose, sulfated 4-position, the remains of which are connected by glycosidic linkages, and correlated with the uronic acid at a ratio of 1: 4 (SO 3 -FUC, UA = COOH), while on the starting material obtained alga Laminaria japonica. The positive effect: low sulfated polysaccharide mole. at. It does not show any toxicity to cells in vitro at doses greater than 5000 ug / mg.

DESCRIPTION OF THE INVENTION

The invention relates to medicine, namely for the prevention and treatment of AIDS and AIDS-related diseases.

Known method the inhibition of HIV infection by fucoidan.

The disadvantage of this method is that the fucoidan-toxic.

The aim of invention is to reduce toxicity.

This goal is achieved by using low-molecular weight sulfated polysaccharide (NSP) with mol.v. about 15,000 daltons, containing fucose, sulfated 4-position, which residues are linked by glycosidic bonds and correlated with uronic acids at a ratio of 1: 4 (SO 3 -FUC; UA-COOH), wherein the starting material is obtained from algae Laminaria japonica ( Laminaria japonica).

CNP has the following characteristics.

Physical description of the polysaccharide. Material and methods.

The chromatographic analysis was performed on a glass column with a hydrophobic sorbent polychrome-1 and Sephacryl S-300 (Pharmacia, Sweden) using Q1 UV-VIS detector (at 254 nm, 2 mL preparative flow cell). Carbohydrates determined by phenol-sulfate method.

For monosaccharide analysis using gas liquid chromatography on 2 Light-chromatograph with a glass column 2.5 x 0.4 cm (3% OV-225 on the chromatron N-HMDS) at about 150-230, about 5 / min.

Samples for analysis were prepared by hydrolysis of a polysaccharide preparation at 90 ° C for 2 hours with 2N HCl, followed by obtaining peracetate algitola conventional manner.

Nuclear Magnetic Resonance Spectrum (H and 13 C NMR) were measured spectrometer Bryker WM-250. The chemical shifts were recorded in ppm from tetrametilksilana.

Results.

NSP (MW about 15,000 Daltons), white amorphous powder; [ ] 25 = 27.813 C NMR (D 20); 102.4; 101.2; 100.7; 99.1 ppm (C-1) 13 C NMR (D 2 O); alginate: 102.4; 101.2; 100.7 (C-1), 71.2; 71.8; 66.0 (C-2), 72.6; 72.8; 70.5 (C-3), 79.2; 801.1 (C-4), 772; 682 (P-5) 176.1; 175.8; 175.6 (C-6) Fucoidan: 99.1 (C-1) 74.9 (C-2), 67.9 (C-3) 81.3 (C-4) 67.9 (C-5) 17.2; 16.6 (C-6).

13 C - NMR spectrum was measured with a spectrometer WM-250 (Bryker).

Thus, it is shown that the polysaccharide fraction with a low molecular weight component of fucose, sulfated 4-position and its remains bound -1,2 Glycosidic bonds and correlated with uronic acids (mannurovoy and guluronic) in the ratio 1: 4 (SO3-FUC: UA-COOH).

The biological characteristics of the polysaccharide.

EXAMPLES EXAMPLE 1 Inhibitory Activity against IPV adsorption of HIV-1 virus and cytopathic effect on the MT4 cells was determined as follows:

a) Direct antiviral effect

1 ml of a suspension of HIV-1 obtained after high speed centrifugation of the virus-containing culture liquid of H9 / IIIB cells or NTN1U 27 and 1 ml of solution corresponding to CNP concentrations (0.1 to 100 .mu.g / ml) was placed in a test tube and allowed to contact for 2 h at 37 ° C or room temperature. Thereafter, an equal number of cells MT4 were pretreated with polybrene added to each tube to adsorb the virus at a multiplicity of infection (MI) of 100, and allowed to contact at 37 ° C for 1-1.5 h.

After contact cells were pelleted by low speed centrifugation for 10 min, the supernatant was removed, the cells were resuspended in RRM1 1640 medium with 10% fetal calf serum to a final concentration of 2 x 10 5 cells / ml. The cell suspension in an amount of 1 ml was dispensed into 24-well plates, and cultured at 37 ° C and 5% CO 2 for 7 days.

b) Inhibitory effect on IPV adsorption of HIV-1 on MT4 cells.

According to 2x10 5 cells / ml MT4 distributed into each well of 24-well plates and after a short period of sedimentation of cells at the bottom of the wells was added the appropriate concentration of CNP and preincubated at 37 ° C for 1 hour, after which was added a virus suspension with a MI-100 and cultured cells under the same conditions seven days. The percentage of cells expressing and neekspressirovavshih viral antigen on the surface membrane was calculated as the average of three replicates of each 100 cells in indirect immunofluorescence method.

The ratio of the X-adsorption inhibition of virus, dependent NSP concentrations were determined by the following formula,%:

where a - antigen positive cells in the preparations treated with NRS,%;

b - antigen positive cells in untreated preparations,%

100 - antigen positive cells in the control formulations (see Tables 1 and 2..).

NRS anticoagulant effect was more than 10 times lower than that of heparin. If the 50% value for the anti-HIV activity in MT4 cells (.., See Tables 1 and 2) transfer of ug / mL IU / ml, we obtain the following values, unit / ml: CNP 6,7h10 -3; fucoidan 3,6h10 -3; heparin 1 x 10-3.

Thus, the NSP has anti-HIV activity at concentrations more than 100-fold lower than its anticoagulant activity.

Determination of in vivo and in vitro acute toxicity of polysaccharide fraction of the NRS.

Determination of acute toxicity in vivo were performed on white mice (10 animals in each group), 4-5 weeks old, weighing 21-24 g NSP diluted in normal saline at appropriate concentrations, was administered to animals in three different ways: intravenously, intraperitoneally, orally. Leading symptoms, mortality and body weight of each animal was investigated for 7-day observation period, after which each animal was sacrificed and the internal organs anatomical study. As shown in Table. 3, none of the animal receiving the drug one of these methods, it was not revealed any signs of acute toxicity or any violations of internal organs morphology (see. Table. 4).

Cytotoxicity EPT in vitro on MT4 and H9 cells was not shown even at drug concentrations above 5000 pg / ml compared to a prototype fucoidan, to which 50% cytotoxic dose determined Survival MT4 cells is 1060 ± 210 pg / ml or azidothymidine ( sigma), which is toxic to cells at concentrations greater than 1,000 micrograms / ml.

Thus, the NSP may be used as a self formula and as a component of a pharmaceutical composition or in combination with other known drugs such as AZT.

The inhibitory effect of EPT on back transcriptase activity in vitro was determined using avian myeloblastosis virus (AMV) as a source of enzyme (provided by the Institute of Molecular Biology and Genetics of the USSR Academy of Sciences, Kiev). Testing was carried out by a known method. NSP 2 mg dissolved in 1 ml of sterile distilled water and adjusted to appropriate concentrations. 20 mL volume of reaction mixture consisted of:

50 mM Tris-HCl, pH 8,2; 5 M MgCl 2; 1 g oligo (d ATP), RNA, pH 8.2 as a template, 20 M dATP, dCTP, dTTP and 0.5 Ki 3 H-dCTP; 60 M KCl, 1 M mercaptoethanol, and placed in a 1.5 ml Eppendorf tube, which was placed in a water bath at 37 ° C for 5 min. Thereafter, 20 l of the previously prepared solution of NSP respective concentrations was added to the reaction mixture together with 1 l of reverse transcriptase AMV and allowed to react at 37 ° C for 1 h. The reaction mixture was transferred to membrane filters DE-81, washed 5 times with 5 -minutnymi break when shaken, dried and placed in glass containers containing 10 ml of liquid scintillation cocktail. Radioactivity (s.r.m.) each filter were counted for 1 min in a scintillation counter. The level of inhibition of reverse transcriptase activities (ITA) was calculated by the formula

ITA = x 100%, where C is about - control the radioactivity of the sample without the NRS;

C s - the radioactivity of the sample after pre-incubation with the NRS.

The level of inhibition on the activity shown in the drawing.

Thus, low sulfated polysaccharide mol.v. It does not show any toxicity to cells in vitro at doses greater than 5000 micrograms / ml, and in vivo in laboratory animals, and can be obtained by using cheap waste technology by treating seaweed (Laminaria japonica).

CLAIM

METHOD inhibition of HIV infection by the use of sulfated polysaccharide, characterized in that, to reduce toxicity, use a low molecular weight sulfated polysaccharide having a molecular weight less than 20,000 daltons, with fucose, sulfated 4-position, the remains of which are linked by glycosidic bonds and correlated with uronic acid at a ratio of 1: 4 (SO 3 - FUC; UA - COOH), wherein the starting material is obtained from algae Laminaria Japanese ( Laminaria japonica).

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Publication date 06.01.2007gg